Emfret公司血小板消除抗體R300說(shuō)明書(shū)

本產(chǎn)品只用于科研，不用于診斷，也不屬于醫(yī)療用品

特異性: This antibody preparation contains a mixture of purified rat monoclonal antibodies  directed

against mouse GPIba (CD42b). Targeting of this receptor with divalent IgGs results in profound and

irreversible Fc-independent plaet depletion in mice^1,2. Plaet depletion is frequently used to study

the general role of plaets in (patho-) physiology³.

制備 : 抗體是雜交瘤細(xì)胞上清通過(guò)G-Sepharose純化所得，內(nèi)毒素含量小于 0.0025 EU/µg

保存和運(yùn)輸：4度保存和運(yùn)輸，保質(zhì)期為6個(gè)月 ，無(wú)菌保存不含防腐劑，分裝保存-20度至少可以保存一年以上，但請(qǐng)避免反復(fù)凍融

使用方法: 按動(dòng)物體重注射抗體，用 量為2 µg抗體/g動(dòng)物體重，可以參見(jiàn)上圖。如果4 µg抗體/g動(dòng)物體重，可以持續(xù)更長(zhǎng)時(shí)間。注射后60分鐘內(nèi)可以去除血小板95%以上，建議每次注射100-200µL抗體（用無(wú)菌PBS稀釋?zhuān)Ｔ斍槁?lián)系靶點(diǎn)科技技術(shù)支持。可以聯(lián)合清除巨噬細(xì)胞（LIPOSOMA，CP-005-005）和中性粒細(xì)胞，咨詢(xún)綜合解決方案。

引用文獻(xiàn):

1. Bergmeier W, Rackebrandt K, Schroder W, Zirngibl H, Nieswandt B. (2000) Structural and functional characterization of the mouse von Willebrand

factor receptor GPIb-IX with novel monoclonal antibodies. Blood. 95:886-93.

2. Nieswandt B, Bergmeier W, Rackebrandt K, Gessner JE, and Zirngibl H. (2000) Identification of critical antigen-specific mechanisms in the

development of immune thrombocytopenic purpura in mice. Blood. 96:2520-27.

3. Elzey BD, Tian J, Jensen RJ et al. (2003) Plaet-mediated modulation of adaptive immunity. A communication link between innate and adaptive

immune compartments. Immunity.19:9-19.